Aromatic hydrocarbons and N-Hydroxymethylacetamide were used as raw materials, seven new acetamide containing capsaicin moieties (A, B, C, D, E, F, G) were synthesized via Friedel-Crafts alkylation reaction and their structures were confirmed by IR and 1H NMR. The bioassay results showed that all the seven compounds exhibited good inhibitioneffect against Staphyloccocus aureus, Escherichia coli, Phaeodactylumtricornu- tum, Skeletonemacostatum and Chaetoceroscurvisetus. In particular, compound G had the best inhibition effect, the minimal antibacterial concentration was 0.0625 mg/mL, and the inhibition rate was over 90% at 3 mg/mL.