10%MOx/Y (M= Mn, Co, Ni, Fe, Ce, Cu) and 5%MnO2-5%MOx/Y (M= Ce, Co, Cu) catalysts prepared by incipient-wetness impregnation method for toluene oxidation with ozone at room temperature. The catalysts were characterized by XRD, BET, H2-TPR and toluene adsorption techniques. The re-sults revealed that 10%MnO2/Y catalyst showed a larger surface area and pore volume, a better tolu-ene adsorption performance, promoting the reaction of toluene oxidation with ozone and the further oxidation of the by-products, thus, 10%MnO2/Y catalyst gave a higher toluene conversion and COx selectivity. With the addition of CuO, the interaction between CuO and MnO2 improved the reduction property of 10%MnO2/Y catalyst and promoted the further oxidation of CO into CO2 with a higher CO2: CO molar ratio. Thus, 5%MnO2-5%CuO/Y catalyst exhibited a high catalytic activity in toluene oxidation with ozone at room temperature with t95 of 240 min, COx selectivity of 80.6% and CO2: CO molar ratio of 4.970. It could be speculated from the results of GC-MS that benzociacid and formic acid phenylmethyl ester as the by-products blocked the catalysts pores and occupied active sites on the surface of the catalysts, resulting in the inactivation of the catalysts.