响应面法优化提取黄檗果实总酚和总黄酮及其抗氧化活性
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中央高校基本科研业务费专项资金项目(2572019BU02,2572015CB18);黑龙江省博士后科研启动金项目(LBH-Q15009);黑龙江省博士后资助经费项目(LBH-Z15006);黑龙江省应用技术研究与开发计划项目(GA19B201);黑龙江省自然科学基金项目(LH2019C006)


Optimization of Extraction of Total Phenolics and Total Flavonoids from Fruits of Phellodendron amurense by Response Surface Method and Their Antioxidant Activity
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Fundamental Research Funds for the Central Universities (2572019BU02); the Heilongjiang Province Postdoctoral Scientific Research Foundation (LBH-Q15009 and LBH-Z15006) and the Natural Science Foundation of Heilongjiang Province (LH2019C006)

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    摘要:

    在单因素实验基础上,根据Box-Behnken实验设计对提取条件进行分析和优化,以考察各因素对黄檗果实总酚(TP)和总黄酮(TF)超声辅助提取率的影响,并进一步研究TP和TF与体外抗氧化活性〔DPPH·清除能力、总自由基清除能力(TRAP)、铁离子还原能力(FRAP)〕的相关性。结果表明,黄檗果实TP和TF最佳超声辅助提取工艺条件为:乙醇体积分数43%,提取温度70℃,pH 值11,超声功率500 W,液固比30:1(mL/g),超声时间40 min,TP(以每克干样品中酚酸类化合物相当于没食子酸GA的质量表示)和TF(以每克干样品中黄酮类化合物相当于芦丁RT的质量表示)得率分别为7.09 ± 0.38 mg GA/g和20.41 ± 0.47 mg RT/g;相关性分析结果表明,TP和TF含量与三种体外抗氧化活性检测结果均为极显著相关(P<0.01),说明TP和TF可能是黄檗果实提取物抗氧化活性的物质基础。

    Abstract:

    On the basis of single-factor test, the extraction conditions of total phenolics (TP) and total flavonoids (TF) from fruits of Phellodendron amurense were analyzed and optimized by the Box-Behnken design, and studied the antioxidant activity of the extracts to determine their correlation. Results showed that, the optimal extraction conditions are as follows: ethanol volume fraction 43%, extraction temperature 72 ℃, pH value 11, ultrasonic power 500 W, liquid/material ratio 30:1 (mL/g), and extraction time 40 min. The yield of TP (expressed as mg of Gallic acid (GA) per gram P. amurense fruit) and TF (expressed as mg of rutin (RT) per gram P. amurense fruit) were 7.09 ± 0.38 mg GA/g and 20.41 ± 0.47 mg RT/g under these conditions. Results showed that there is a significant correlation between in vitro antioxidant activity and contents of TP and TF(P<0.01).and TF(P<0.01). This work would provide a basis for the further development of P. amurense.

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陈小强,刘玲玲,孙彤彤,张莹.响应面法优化提取黄檗果实总酚和总黄酮及其抗氧化活性[J].精细化工,2020,37(2):

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  • 收稿日期:2019-06-21
  • 最后修改日期:2019-08-20
  • 录用日期:2019-08-23
  • 在线发布日期: 2020-01-10
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