In order to develop the flavor precursor of pyrazine ester, 2,5-dimethylpyrazine N-oxide and thiophene methyl acrylate were used as raw materials, and the target compound thiophen-2-ylmethyl-3-(3,6-dimethylpyrazine-2-yl)acrylate N-oxide was synthesized by coupling reaction with the catalysis of palladium acetate. The target compound was characterized by 1H NMR, 13C NMR, IR, HRMS, and its thermal property was measured by thermogravimetric-differential thermogravimetry (TG-DTG) and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). The results show that degradation temperature of the target compound is at 170 oC, and the main thermal weight loss range is 170 oC-875 oC. The maximum weight loss rate is 257 oC, and the total weight loss is 76.5%. Under the conditions of inert and oxidative atmosphere at 300 oC-900 oC, 11 volatile products are identified, and the main characteristic aroma compounds are alkylpyrazines, 2-methylthiophene and 2-thiophene methanol. In the oxidative atmosphere, there are more types of pyrolysis products, and their relative contents are higher than in inert atmosphere. According to the types and relative contents of the main pyrolysis products, the possible pyrolysis mechanism of the target compound is revealed. The optimum addition amount of the target compound in cigarette is 2 mg/kg.