Due to its low price and availability, good hemostasis and biocompatibility, chitosan (CS) and its derivatives are widely used in the development of antibacterial and hemostatic materials. Alkylated chitosan (N-CS) was prepared by N-alkylation modification of CS with dodecanal and embedding N-CS into the polydopamine-modified graphene oxide (DGO) framework in acetic acid solution to prepare novel hemostatic powders (N-CS/DGO). The structure and morphology were characterized by FTIR, SEM and BET, which indicated that the surface of N-CS/DGO hemostatic powder contained a large number of active groups, and had excellent hydrophilicity, large specific surface area and rich pore structure. When DGO account for 15%, the composite power had the best potential for hemostasis. At this time, the material could absorb water by 430% over itself , and the degradation ratio was appropriate, reaching 88% in five weeks. The inhibition zones for E. coli and S. aureus were 1.92 mm and 1.98 mm, respectively. With certain bacteriostatic ability, the coagulation index could reach its minimum, that was 32%, and the in vitro coagulation time was shortened to 126± 6 s, all of which indicated that N-CS / DGO materials had potential for hemostasis.