In order to study the effect of mixing condition on the thermo-sensitivity of chitosan/ sodium β-glycerophosphate (β-GP) solutions. The β-GP solution (50 wt%) was added dropwise in the chitosan solution(2 wt%), and the viscoelastic properties of the solutions were studied by off-line rheology. It is found that the system has thermal induced sol - gel transition character when the volume ratio of chitosan solution/β-GP solution is 8/2. When more β-GP solution was added, the sol-gel transition temperatures of the chitosan/β-GP solutions are similar, but the gel structure loose. Temperature is the important influence factor for the chitosan/β-GP solution. The solution is stable at the temperature of 4 °C and forms weak gel at 25 °C after a certain time. When the temperature is above 30 °C, sol-gel transition occurs quickly.