Using fish skin gelatin (FG) and carboxymethyl chitosan (CMCS) as raw materials, orange essential oil (OEO) as antibacterial agent, the antibacterial composite membrane was prepared by crosslinking method, through FTIR, XRD, SEM, thermal weight analysis, and its mechanical, optical, barrier, antibacterial properties were tested.the results show that, Better composite of the OEO with the FG / CMCS basement membrane, Compared with the FG / CMCS basement membrane, When added (measured by the weight of basement membrane forming fluid, The same below) is 0.8%, The tensile strength of the composite membrane was reduced by 10.46%, But it still has good mechanical properties, The elongation rate of fracture increased by 12.96%; A decline in transparency, Light transmittance decreased by 8.7%; Water resistance and water vapor barrier performance are significantly enhanced, Water content, water solubility and water vapor transmittance decreased by 22.48%, 30.49% and 26.41%, respectively; DPPH radical clearance increased by 247.88%, The oxidation resistance is significantly improved; It has a good antibacterial effect against E. coli and S. aureus.