In order to remove residual liquid crystals in the liquid crystal box to improve the performance of the liquid crystal display, a series of Gemini surfactants BNPA-H-400, BNPA-F-200, BNPA-F-400, and BNPA-F-500 were synthesized using polyethylene glycol monomethyl ether and bisphenol compounds as raw materials. The structural configurations of these surfactants were confirmed through comprehensive analytical characterization including FTIR,1HNMR, 13CNMR, 19FNMR, and LC-QQQ-MS.Their surface activity, wetting performance, low-foaming properties, and emulsification capability were systematically evaluated. The results show that the overall performance of BNPA-F-400 is the best. BNPA-F-400 was mixed with ethanolamine, alcohols, C8-10, ethoxylated (JFC-6), and water as a water-based cleaning agent, and its application in liquid crystal cleaning was studied. When the optimal mass fractions of BNPA-F-400, ethanolamine, JFC-6, and water in the water-based cleaning agent are 0.5%, 5%, 3%, and 91.5%, respectively, the cleaning efficiency of the liquid crystal is the best, reaching 98.77%. Compared with commercially available nonionic surfactants alcohols, C12-18, ethoxylated (AEO-9), polyethylene glycol lauryl ether (O-20), and polyethylene glycol mono(4-tert-octylphenyl) ether (TX-10), the cleaning efficiency of BNPA-F-400 was improved by 2.63%, 1.56%, and 1.57%, respectively.