A series of novel aromatic alkylamine compounds were designed and prepared from the starting materials of 1,1'-(4-chlorobutylidene)bis(4-flourobenzene) and dibenzoic acid through a serious of coupling reaction, and the structures were characterized by 1H NMR,13C NMR and MS. Biological evalua- tion in vitro were evaluated by human prostate cancer cells (DU145、PC3) , breast cancer cells (MCF-7、MDB-MB-231、MDB-MB-453) and 926 by MTT assay．The results indicates that T3 has the best biological activity for MDB-MB-231(IC50=17.30μM) as well as T4 (IC50=19.07μM). However, the IC50 of 926 cells were shown the high toxicity on normal epithelial cells (IC50: 19.20, 26.37, μM). Compounds T7 had inhibitory effect against breast cancer cells(IC50: 52.03, 49.74,30.09, μM) and had a low toxicity on 926 (IC50>50).