Ultrasonic oxidation stripping method was applied to preparation graphene oxide (GO). The cotton fabrics padding with GO, then drying the GO padded cotton fabrics, finally the reduced graphene oxide (RGO) coated electric conductive cotton fabrics was obtained by chemical reduction. The influence of GO concentration, pH value, reduction condition, padding-reduction times on electrical conductivity of cotton fabrics was researched. The cotton samples were characterized by SEM and Raman spectra. The washing fastness of the samples was also tested. The results showed that the optimal conductive finishing process, GO 2.5 g/L, pH value of GO solution was controlled about 6, sodium hydrosulfite 5g/L, reduced at 95℃ for 30 min, the surface electric resistance of cotton after 1, 4 and 8 times of padding-reduction process was decreased to 2220 Ω, 270 Ω and 130 Ω, respectively. The SEM results showed that after padding with GO, GO forms a thin film on the surface of the fabric. The Raman results showed that the GO was deposition on the surface of cotton fabrics, and the GO fully reduced to RGO. The result of washable property of electric conductive cotton fabric was found that the conductive durability of the treated fabric is excellent.