Herein, we reported an efficient method to manufacture 2'-Deoxycytidine. This process, which is using α-D-2-Deoxyribose and Uracil as economical starting material, went through 6 linear steps, including methyl-glycosidation, protection, chlorination, nucleophilic substitution, amination and deprotection reactions. The final product is obtained as 44% overall yield and 99% purity after recrystallization. This process has several advantages, such as mild reaction conditions, relative safe operation and straightforward purification for final product and intermediates. Up to now, kg-scale manufacture of 2-Deoxycytidine is completed in the chemical factory.